[HTML][HTML] Role of protein targeting to glycogen (PTG) in the regulation of protein phosphatase-1 activity
MJ Brady, JA Printen, CC Mastick, AR Saltiel - Journal of Biological …, 1997 - ASBMB
We have recently cloned from 3T3-L1 adipocytes a novel glycogen-targeting subunit of
protein phosphatase-1, termed PTG (Printen, JA, Brady, MJ, and Saltiel, AR (1997) Science
275, 1475–1478). Differentiation of 3T3-L1 fibroblasts into highly insulin-responsive
adipocytes resulted in a marked increase in PTG expression. Immobilized glutathioneS-
transferase (GST)-PTG fusion protein specifically bound either PP1 or phosphorylase a.
Addition of soluble GST-PTG to 3T3-L1 lysates increased PP1 activity against 32 P-labeled …
protein phosphatase-1, termed PTG (Printen, JA, Brady, MJ, and Saltiel, AR (1997) Science
275, 1475–1478). Differentiation of 3T3-L1 fibroblasts into highly insulin-responsive
adipocytes resulted in a marked increase in PTG expression. Immobilized glutathioneS-
transferase (GST)-PTG fusion protein specifically bound either PP1 or phosphorylase a.
Addition of soluble GST-PTG to 3T3-L1 lysates increased PP1 activity against 32 P-labeled …