Measurement of protein sulfenic acid content
LB Poole - Current Protocols in Toxicology, 2008 - Wiley Online Library
LB Poole
Current Protocols in Toxicology, 2008•Wiley Online LibraryProtein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox
catalysis and regulation. Detection and quantification of these species in proteins is
accomplished through chemical modification by reagents such as 7‐chloro‐4‐nitrobenzo‐2‐
oxa‐1, 3‐diazole (NBD chloride), 2‐nitro‐5‐thiobenzoate (TNB), dimedone, or derivatives of
dimedone, followed by UV‐visible spectroscopy or mass spectrometric analysis. Curr.
Protoc. Toxicol. 38: 17.2. 1‐17.2. 27.© 2008 by John Wiley & Sons, Inc.
catalysis and regulation. Detection and quantification of these species in proteins is
accomplished through chemical modification by reagents such as 7‐chloro‐4‐nitrobenzo‐2‐
oxa‐1, 3‐diazole (NBD chloride), 2‐nitro‐5‐thiobenzoate (TNB), dimedone, or derivatives of
dimedone, followed by UV‐visible spectroscopy or mass spectrometric analysis. Curr.
Protoc. Toxicol. 38: 17.2. 1‐17.2. 27.© 2008 by John Wiley & Sons, Inc.
Abstract
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD chloride), 2‐nitro‐5‐thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV‐visible spectroscopy or mass spectrometric analysis. Curr. Protoc. Toxicol. 38:17.2.1‐17.2.27. © 2008 by John Wiley & Sons, Inc.
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