Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
Nature methods, 2014•nature.com
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells
grown in serum or in 2i medium displayed epigenetic heterogeneity, with'2i-like'cells present
in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the
whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation
in rare cells and heterogeneous populations.
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells
grown in serum or in 2i medium displayed epigenetic heterogeneity, with'2i-like'cells present
in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the
whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation
in rare cells and heterogeneous populations.
Abstract
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
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