[HTML][HTML] Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway
A Fukunaka, Y Kurokawa, F Teranishi, I Sekler… - Journal of Biological …, 2011 - ASBMB
A number of enzymes become functional by binding to zinc during their journey through the
early secretory pathway. The zinc transporters (ZnTs) located there play important roles in
this step. We have previously shown that two zinc transport complexes, ZnT5/ZnT6
heterodimers and ZnT7 homo-oligomers, are required for the activation of alkaline
phosphatases, by converting them from the apo-to the holo-form. Here, we investigated the
molecular mechanisms of this activation. ZnT1 and ZnT4 expressed in chicken DT40 cells …
early secretory pathway. The zinc transporters (ZnTs) located there play important roles in
this step. We have previously shown that two zinc transport complexes, ZnT5/ZnT6
heterodimers and ZnT7 homo-oligomers, are required for the activation of alkaline
phosphatases, by converting them from the apo-to the holo-form. Here, we investigated the
molecular mechanisms of this activation. ZnT1 and ZnT4 expressed in chicken DT40 cells …
