Fluorometric evidence for the binding of cholesterol to the filipin complex
F Schroeder, JF Holland, LL Bieber - The Journal of antibiotics, 1971 - jstage.jst.go.jp
F Schroeder, JF Holland, LL Bieber
The Journal of antibiotics, 1971•jstage.jst.go.jpMaterials and Methods Filipin, 86% pure, was kindly provided by the Upjohn Company,
Kalamazoo, Michigan. The complex was further purified by washing twice with chloroform
and twice with petroleum ether. After drying, the filipin complex was dissolved in tertiary butyl
alcohol, lyophilized, and stored in the dark at-20 C. The standard filipin solution was
prepared by dissolving 1mg in 100ml distilled water. Partial quantum efficiency (PQ), correct
fluorescence(CO), and absorption spectra were determined as decribed by Holland and
Kalamazoo, Michigan. The complex was further purified by washing twice with chloroform
and twice with petroleum ether. After drying, the filipin complex was dissolved in tertiary butyl
alcohol, lyophilized, and stored in the dark at-20 C. The standard filipin solution was
prepared by dissolving 1mg in 100ml distilled water. Partial quantum efficiency (PQ), correct
fluorescence(CO), and absorption spectra were determined as decribed by Holland and
Materials and Methods
Filipin, 86% pure, was kindly provided by the Upjohn Company, Kalamazoo, Michigan. The complex was further purified by washing twice with chloroform and twice with petroleum ether. After drying, the filipin complex was dissolved in tertiary butyl alcohol, lyophilized, and stored in the dark at-20 C. The standard filipin solution was prepared by dissolving 1mg in 100ml distilled water. Partial quantum efficiency (PQ), correct fluorescence(CO), and absorption spectra were determined as decribed by Holland and
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