Cholesterol efflux-mediated signal transduction in mammalian sperm: β-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine …
PE Visconti, H Galantino-Homer, XP Ning… - Journal of Biological …, 1999 - ASBMB
Sperm capacitation in vitro is highly correlated with an increase in protein tyrosine
phosphorylation that is regulated by cAMP through a unique mode of signal transduction
cross-talk. The activation of this signaling pathway, as well as capacitation, requires bovine
serum albumin (BSA) in the incubation medium. BSA is hypothesized to modulate
capacitation through its ability to remove cholesterol from the sperm plasma membrane.
Here we demonstrate that the cholesterol-binding heptasaccharides, methyl-β-cyclodextrin …
phosphorylation that is regulated by cAMP through a unique mode of signal transduction
cross-talk. The activation of this signaling pathway, as well as capacitation, requires bovine
serum albumin (BSA) in the incubation medium. BSA is hypothesized to modulate
capacitation through its ability to remove cholesterol from the sperm plasma membrane.
Here we demonstrate that the cholesterol-binding heptasaccharides, methyl-β-cyclodextrin …
