[HTML][HTML] Evidence that the major oxysterols in human circulation originate from distinct pools of cholesterol: a stable isotope study
The major oxysterols in human circulation are 7α-, 27-, and (24S)-hydroxycholesterol. Two
unique experiments were performed to elucidate their origin and kinetics. A volunteer was
exposed to 18 O 2-enriched air. A rapid incorporation of 18 O in both 7α-and 27-
hydroxycholesterol and disappearance of label after exposure were observed. The half-life
was estimated to be less than 1 h. Incorporation of 18 O in (24S)-hydroxycholesterol was not
significant. In the second experiment a volunteer was infused with liposomes containing 10 …
unique experiments were performed to elucidate their origin and kinetics. A volunteer was
exposed to 18 O 2-enriched air. A rapid incorporation of 18 O in both 7α-and 27-
hydroxycholesterol and disappearance of label after exposure were observed. The half-life
was estimated to be less than 1 h. Incorporation of 18 O in (24S)-hydroxycholesterol was not
significant. In the second experiment a volunteer was infused with liposomes containing 10 …
